Goals from Craig Curran and Gareth Edds gave Tranmere their third home league win in a row and lifted them up to 22nd in League One.
The win was the reward for the hard work Les Parry's side showed throughout, with some strong defending at the back from Ian Goodison and Ash Taylor and further chances to score at the other end.
There was just one change to the Tranmere side with Paul McLaren replacing the suspended Marlon Broomes as Ash Taylor moved into the centre of defence.
Shaleum Logan had the game's first shot, but the defender's first time effort from 25 yards went over the opposition bar.
Ian Thomas-Moore forced a decent save out of Mikkel Andersen with a trademark volley following good work from Terry Gornell, while at the other end Luke Daniels produced a one handed save to keep out Mark Wright's back post volley.
Daniels was almost caught out on 13 minutes when racing out of his area to clear a long ball forward from the Bristol Rovers defence. The ball bounced over the Tranmere keeper but he was quick to turn and gather before Chris Dickson could pounce.
Tranmere came inches from taking the lead from another training ground set piece routine on 18 minutes. The ball was played to Zoumana Bakayogo who raced forward from the halfway line and his cross found Ian Goodison only for the Jamaican international to head narrowly wide of Andersen's right hand post.
On 23 minutes a cross from McLaren landed on the roof of the Pirates' net and Gornell sliced a shot from 25 yards as Tranmere looked for the opener.
The goal came on 29 minutes and it arrived in spectacular fashion. Curran picked the ball up just outside the area from Logan and unleashed a perfect curling shot with his left foot into the top left corner - his fifth goal of the season.
A 25 yard effort from Gornell deflected behind for a corner on 38 minutes - the last effort of the half as Tranmere took their lead into the break.
Les Parry's side&nbsp;survived a scare on 55 minutes when Andy Williams' header hit the bar and bounced back into play, with John Welsh eventually clearing the danger.
It was made to look more significant moments later when Tranmere doubled their lead. After good work from Gornell, Bakayogo's cross from the left was flicked on by Ian Thomas-Moore to Gareth Edds who controlled the ball before firing low past Andresen from 12 yards.
Another well worked free kick saw Bakayogo float the ball in to the unmarked Curran on 63 minutes but his header went straight at Andresen.
Edds tried for a second on 64 minutes but his 20 yard first time shot from Thomas-Moore's knock down went wide of the target, and the midfielder went even closer five minutes later with a low effort from the edge of the area in a quick counter attack.
On 70 minutes some quick thinking by Logan released Curran up the right wing and his low cross found Thomas-Moore at the far post. His shot beat Andresen only to be cleared off the goal line by Steve Elliott.
A minute later Gornell raced clear up the left but chose to shoot when a pass to the unmarked Edds would have been the better option.
Appeals for a penalty were ignored on 76 minutes as Thomas-Moore closed down Andresen and went down as the keeper attempted to clear a back pass.
Curran fired narrowly wide of the left post on 80 minutes after Edds won the ball in midfield while Logan and Taylor threw themselves in the way of Chris Lines' shot at the other end six minutes later.
Six minutes of&nbsp;injury time was added on following a delay for treatment to Elliott just before 90 minutes, with the defender being forced to come off and the visitors&nbsp;left to finish the game with ten men.
Daniels produced a fine save to keep out Darryl Duffy's late effort, a stop that earned the keeper a clean sheet. &nbsp;
Tranmere: Daniels, Logan, Taylor, Goodison, Bakayogo, Edds, McLaren, Welsh, Curran, Gornell, Thomas-Moore. Substitutes not used: Collister, Cresswell, Barnett, Fraughan, Ricketts, Shuker, Macauley.
Bristol Rovers: Andersen, Regan, Wright (Swallow 64), Lines, Elliott, Campbell, Williams, Dickson (Duffy 64), Hughes, Lescott (Blizzard 82), Baldwin. Substitutes not used: Evans, Coles, Anthony, Reece.
Referee: Jarnail Singh
Yellow cards: Lescott (62), Hughes (80)