Ash Taylor's first goal for the Club earned Rovers a 1-0 win over Leyton Orient and a place in the Second Round of the FA Cup.
The youngster netted late on after Rovers had defended strongly against the home side, with his effort setting up a home tie against Aldershot Town in the next round.
There were three changes made to the side that started at Norwich. Charlie Barnett and Shaleum Logan returned from suspension and Ian Thomas-Moore came back in, while John Welsh missed out as a result of a one match ban and Ryan Fraughan and Michael Ricketts dropped to the bench. The changes meant Ash Taylor moved into the centre of midfield with Marlon Broomes returning to the centre of defence alongside Ian Goodison. Former Rovers players Ben Chorley and Sean Thornton started for Orient.
The home side enjoyed the better of the early stages, with Luke Daniels forced into a number of saves, though none of them overly tested the abilities of the West Brom loanee.
Rovers appealed for handball and a penalty on 19 minutes but referee Andy Woolmer waved play on while Daniels saved from Scott McGleish at the other end.
Daniels caught Tamika Mkandawire's overhead kick from a corner on 36 minutes and Logan did well to clear a minute later as the hosts pressed for an opener. Next to aim at goal was Jason Demetriou but again Daniels saved comfortably as Rovers held firm.
Chorley drilled a free kick against the Tranmere wall from a dangerous position in front of goal on 44 minutes, the last chance of a goalless half.
Taylor put a shot over the bar from outside the area on 49 minutes for Rovers while Daniels saved from JJ Melligan three minutes later and Thornton curled over the Tranmere goal soon after from a free kick.
Daniels produced another good save to deny substitute Ryan Jarvis and Barnett forced Glenn Morris into a decent stop on 76 minutes with a powerful effort from the edge of the area.
Rovers began to push forward with Taylor firing wide from 18 yards and the youngster broke the deadlock with just eight minutes remaining, firing in from close range after the Orient defence failed to clear a Tranmere free kick.
A couple of minutes later Orient thought they had levelled but Scowcroft's looped effort was ruled out for a foul on Daniels to the relief of the travelling fans.
Thomas-Moore sent a shot at Morris two minutes before the end of normal time and though the home side threw men forward in the final they were unable to find a way through to the Tranmere goal as Rovers progressed to the next round.
Leyton Orient: Morris, Purches (Patulea 86), Chorley, Mkandawire, Melligan (Jarvis 64), Chambers, McGleish, Thornton, Demetriou, Daniels, Scowcroft. Substitutes not used: Ashworth, Jones, Cave-Brown, Pires, Smith.
Tranmere: Daniels, Logan, Broomes, Goodison, Bakayogo, McLaren, Taylor, Barnett, Curran, Thomas-Moore, Shuker. Substitutes not used: Collister, Cresswell, Fraughan, Gornell, Ricketts, Bain, Baker.
Referee: Andy Woolmer
Yellow cards: Logan (63), Thomas-Moore (64), Taylor (90)
Away attendance: 145