Second half goals from Will Grigg and Craig Westcarr gave Walsall a 2-0 win over Rovers at the Banks's Stadium on Tuesday night.
After a fairly even first half, the hosts took the lead from the penalty spot soon after half time and addded the second on the hour mark to claim all three points.
There was one change to the team that started against Oldham with Mamady Sidibe returning from injury to take the place of Paul Corry. Max Power reverted to the centre of midfield with Michael O'Halloran dropping into right midfield.
Rovers created the first chance of the game in the second minute with Liam Palmer cutting past James Chambers before bringing a save out of Aaron McCarey at his near post.
The Walsall keeper was called on again two minutes later. A stepover from O'Halloran allowed the ball to run to Jean-Louis Akpa Akpro and his cross was neatly brought down by Sidibe 12 yards out and his volley was caught by McCarey.
It needed a goal line clearance from Ash Taylor to keep out Jamie Paterson's header as Walsall almost took the lead from a set piece on five minutes while another Sidibe effort had to be saved by McCarey.
Walsall's Will Grigg beat the offside trap on 11 minutes and from his cross Andy Butler saw a header saved by Fon Williams at his back post.
A flick on from Sidibe released O'Halloran on the left on 23 minutes and the Bolton Wanderers loanee raced past Andy Taylor and into the area before his run was ended by a superb tackle from Chambers.
Fon Williams had to dive to his left to keep out Paterson's first time side foot shot on 28 minutes after the winger ran on to Ian Goodison's headed clearance.
The home side went even closer two minutes later with former Rovers loanee Sam Mantom sending a shot from 25 yards through a crowded area and against the left hand post, with Goodison clearing the rebound.
Paterson shot over from a Febian Brandy cross on 32 minutes, the final chance of the first half.
Chambers twice had to block shots from Abdulai Bell-Baggie two minutes after the restart but soon after the home side took the lead. Brandy raced past challenges from Goodison and Taylor before being brought down by Holmes inside the area. Referee Graham Salisbury pointed straight to the penalty spot and Will Grigg shot into the bottom right corner past the outstretched arm of Fon Williams to put Walsall 1-0 up.
Craig Westcarr brought a save out of Fon Williams from outside the area on 53 minutes with the Rovers keeper gathering at the second atttempt but the striker's next effort doubled Walsall's advantage. The former Chesterfield forward found the net from outside the area with a fantastic strike past Fon Williams that gave the keeper no chance of a save.
After McCarey spilled a free kick from Bell-Baggie and claimed at the second time of asking Akpa Akpro saw a shot blocked inside the area.
A long range effort from Butler went over the bar in the 90th minute, the final chance of the game at either end.
Walsall: McCarey, Butler, Chambers, Grigg, Paterson, Brandy (Featherstone 84), Downing, Chambers, Taylor, Mantom, Westcarr. Substitutes: Holden, Hemmings, Purkiss, Bowerman, Baxendale, Roberts.
Tranmere: Fon Williams, Holmes, Taylor, Goodison (Daniels 56), Bakayogo, O'Halloran (Stockton 64), Power, Palmer, Bell-Baggie (Amoo 81), Akpa Akpro, Sidibe. Substitutes: Mooney, Black, Corry, Harrison.
Referee: Graham Salisbury
Yellow cards: Holmes (51), Brandy (60), Westcarr (60), Power (60), Bell-Baggie (77)