Goals from Jean-Louis Akpa Akpro and Ryan Lowe gave Tranmere a 2-1 win over Colchester United at the Weston Homes Community Stadium.
The pair scored either side of half time, with Lowe's strike clinching the victory and lifting Rovers to 43 points.
There were two changes for Tranmere, as Stephen Arthurworrey and Jason Koumas returned from injury to be named in the starting eleven.
With the referee refusing to allow Tranmere to play in their sky blue away kit, Rovers were forced to wear Colchester's gold third kit.
Jabo Ibehre fired over Owain Fon Williams' goal from the edge of the area in the second minute and the striker fired straight at the keeper three minutes later after running through on goal from the halfway line.
At the other end Jean-Louis Akpa Akpro broke past a defender and played the ball across the six yard box but the Colchester defence cleared before it reached Max Power at the back post.
Akpa Akpro shot wide from 20 yards after controlling a lay off from Ryan Lowe on his chest and the pair linked up again on 13 minutes, with Lowe's right foot shot being turned behind for a corner.
Alex Gilbey headed over from a corner on 14 minutes and Fon Williams produced a stunning one handed save to keep out a volley from the midfielder two minutes later.
Fon Williams tipped a Freddie Sears shot over the bar on 21 minutes while Lowe side footed into the hands of Sam Walker at the other end after running on to a pass from Akpa Akpro.
Ibehre's attempted lob was caught by Fon Williams on 24 minutes and as the action continued to swing from one end to the other, Ash Taylor brought a save out of Walker with a low strike from 30 yards.
Fon Williams reacted well to push Ibehre's shot away from goal following a scramble on 33 minutes and three minutes later Rovers took the lead.
Power picked up possession inside his own half and with a run from Lowe dragging defenders to one side, the midfielder picked out the run of Akpa Akpro on the left. The striker controlled, ran towards the keeper and slotted a low shot into the bottom right corner from 12 yards.
The hosts equalised just before half time. Vose played the ball to Ryan Dickson inside the area and his cut back was turned into the bottom right corner by Sears.
Rovers started the second half brightly, winning a couple of corners in quick succession before Jake Cassidy curled wide of the near post.
Walker saved from Lowe on 52 minutes while Rovers' first change saw Danny Holmes replace Stephen Arthurworrey a minute later, with Matthew Pennington moving to centre back.
Jake Kirby came on for Jason Koumas on 58 minutes and the young midfielder volleyed over from the edge of the area two minutes later following a corner.
On 62 minutes Tranmere regained the lead. Akpa Akpro used his pace to get past Massey and into the area and though his shot was blocked the ball fell to Lowe at the back post who stroked into the net for his 20th goal of the season.
A volley from Massey deflected wide of goal on 72 minutes and Ridehalgh headed clear a Ryan Dickson free kick away from goal soon after.
Cassidy got between the keeper and a defender on 77 minutes but his shot hit Alex Wynter before being cleared.
Cole Stockton replaced Lowe on 86 minutes and the striker had to help out with the defensive effort in the dying stages as Colchester pushed for an equaliser.
On 90 minutes a 20 yard strike from substitute Drey Wright beat Fon Williams but Pennington headed clear off the line to the relief his team mates and the 145 travelling supporters.
Colchester: Walker, Olufemi (Watt 45 HT), Okuonghae, Eastman, Dickson, David Wright (Drey Wright 83), Gilbey, Vose (Wynter 68), Massey, Sears, Ibehre. Unused substitutes: Cousins, Morrison, Bean, Szmodics.
Tranmere: Fon Williams, Pennington, Taylor, Arthurworrey (Holmes 53), Ridehalgh, Akpa Akpro, Power, Jennings, Koumas (Kirby 58), Cassidy, Lowe (Stockton 86). Unused substitutes: Mooney, Boland, Brown, Ariyibi.
Referee: Andy Davies
Yellow cards: Olufemi (19), Arthurworrey (49), Power (87)