Tranmere's hopes of staying in League 1 will go down to the final day of the season after a 2-0 defeat at Leyton Orient.
Rovers conceded shortly before half time and despite improving early in the second half, a long range strike from Chris Dagnall sealed the points for the hosts.
Jean-Louis Akpa Akpro and Liam Ridehalgh recovered from injury to take their places in the starting line-up while Ian Goodison and Abdulai Baggie returned to the substitutes' bench after spells on the sidelines.
Ryan Lowe curled over in the first minute after being found by Junior Brown on the edge of the area while Owain Fon Williams did well to keep out Dean Cox's 25 yard strike at the other end soon after.
Orient dominated possession for large periods early on but without creating any clear openings. A spell of Rovers dominance saw the visitors win a couple of corners in quick succession around the 35 minute mark though Orient broke quickly from the second with winger Shaun Batt firing into the side netting from 15 yards.
The home side took the lead a minute from half time. After Matthew Pennington had blocked a shot from David Mooney, Scott Cuthbert's header from the corner was saved by Fon Williams but Kevin Lisbie reacted quickly to turn in from close range.
James Rowe and Jake Kirby replaced Jean-Louis Akpa and Cassidy for the second half and Rovers started strongly.
Ash Taylor's flick from a Rowe corner was saved by Jones and the keeper held on to a curling effort from Max Power on 50 minutes.
Junior Brown turned quickly on to Rowe's pass on 55 minutes only to see his shot blocked while Orient's Mooney sent a headed clearance only inches wide of his own goal soon after.
Moses Odubajo brought a fantastic one handed save out of Fon Williams with a low strike from the edge of the area and the winger side stepped Taylor and Pennington before firing straight at the keeper on 74 minutes.
Orient's second goal came on 76 minutes. The hosts pressed and forced Steve Jennings to play the ball back to Fon Williams. His clearance landed straight at the feet of former Rovers striker Chris Dagnall who drilled a shot into the empty net from 35 yards.
Kirby fired across goal and wide from a tight angle after good hold up play from Rowe on 86 minutes while Power's long range strike was fumbled by Jones in added time.
The Orient keeper held on to a late effort from Ridehalgh as Rovers went home empty handed.
Leyton Orient: Jones, Sawyer, Vincelot, Cuthbert, Cox, Lisbie (Dagnall 70), Mooney, Odubajo, Batt (James 59), Clarke, Lundstram (Ness 81). Unused substitutes: Larkins, Lasimant, Gorman, Simpson.
Tranmere: Fon Williams, Holmes, Taylor, Pennington, Ridehalgh, Brown, Power, Jennings, Akpa Akpro (Rowe 45 HT), Cassidy (Kirby 45 HT), Lowe. Unused substitutes: Mooney, Goodison, Arthurworrey, Baggie, Stockton.
Referee: Darren Sheldrake
Yellow cards: Jennings (29), Fon Williams (68), Lowe (68), Cuthbert (68)