Tranmere fell to a 2-1 defeat at the hands of promotion-chasing Wycombe Wanderers at Prenton Park.
All the goals came in the first 45 minutes with Paul Hayes opening the scoring for Wycombe from the penalty spot. Jordan Hugill levelled on his home debut but Fred Onyedinma's 40th minute strike turned out to be the winner for the visitors.
Wycombe did have Matthew McClure sent off with 22 minutes remaining but despite a lot of Tranmere pressure towards the end, it was not enough and the Chairboys held on for the victory.
Tranmere manager Micky Adams named two changes to the side which started at Cheltenham Town on Saturday with Harrison McGahey and Marc Laird replacing injured duo Jennison Myrie-Williams and Rory Donnelly in a 3-5-2 formation. Dan Gardner, who joined on loan from Chesterfield, was named on the substitutes' bench.
The visitors had the first opportunity of the evening on nine minutes as a cross from the left wing was only half cleared but Sam Saunders' effort from the edge of the box was too high and flew over the crossbar.
Ten minutes later, Wycombe had the first shot on target of the game as Joe Jacobsen's free-kick from the right ended up at the feet of Alfie Mawson whose shot was saved by Owain Fon Williams before Sam Saunders fired a free-kick from 20 yards over the bar.
Midway through the first half, Lee Molyneux sent over a wicked cross from the left which narrowly evaded Jordan Hugill in the penalty area but the former Crewe man volleyed over the crossbar moments later.
The opening goal came for Wycombe on 26 minutes from the penalty spot. Harrison McGahey was adjudged to have handled in the area and Paul Hayes converted from 12 yards past the despairing dive of Fon Williams.
Tranmere equalised six minutes later after a spell of pressure. Max Power had put in a couple of dangerous crosses from the right earlier on but his third was met by Hugill eight yards out and the striker diverted the ball into the net for his first goal for the club.
The Chairboys restored their lead on 40 minutes as Fred Onyedinma beat the offside trap, burst through on goal and slid the ball underneath Fon Williams.
Rovers began the second half brightly and, after a couple of dangerous crosses went into the box, Laird had a volley from the edge of the penalty area which was well blocked and cleared by the Wycombe defence.
On 57 minutes, Hugill went looking for a second goal as he ran on to a forward ball but he couldn't control his shot and it flew wide of the target.
Micky Adams made a double change on the hour mark, handing Dan Gardner his debut in place of Harrison McGahey while Iain Hume replaced goalscorer Jordan Hugill.
Midway through the second half, Hume's first contribution was to break clear down the right but his low cross was cleared away before a deep cross by Power found Steve Jennings at the back post but his shot was blocked and cleared to safety by the Wycombe defence.
Wycombe were reduced to 10 men on 68 minutes when substitute Matt McClure was sent off for a clash with Adam Dugdale.
With 18 minutes remaining, Tranmere made their final change with Jake Kirby introduced in the place of Jennings.
A deep cross from Danny Holmes on 82 minutes found Gardner at the back post who turned inside and fired a low shot which Ingram saved at his near post.
In the final embers of the game, a Power corner was met by the head of Michael Ihiekwe but his effort was cleared off the line by Sam Wood before substitute Kirby fired a powerful shot into the hands of Ingram in the Wycombe goal but it was not enough as the visitors held on to take all three points.
Tranmere: Fon Williams, Holmes, McGahey (Gardner 60), Dugdale, Ihiekwe, Molyneux, Power, Jennings (Kirby 72), Laird, Odejayi, Hugill (Hume 60). Substitutes: Ramsbottom, Ridehalgh, Thompson, Koumas.
Wycombe: Ingram, Jombati, Jacobson, Hayes, Rowe, Mawson, Bloomfield (Murphy 89), Wood, Onyedinma (Craig 89), Yennaris, Saunders (McClure 67). Substitutes: Richardson, Ephraim, Holloway, Fletcher, McClure.
Referee: Mark Heywood
Yellow cards: Hugill (36), Odejayi (69), Ihiekwe (90+5)
Red card: McClure (68)