Rovers fans Antony Philips, Mike Jones and Mark Lewis are making a special trip to the Crewe match on Saturday.
The trio, who are pupils at Caldy Grange Grammar Sixth Form, are being sponsored to walk from Wirral to Crewe ahead of the match. They will leave at around midnight on Friday for the 40 mile journey.
They are raising money for the Sam Bencheikh Memorial Fund, which helps raise money for Tranmere Rovers In The Community, the Club's charity.