Tranmere Reserves suffered a 4-0 away defeat in the totesport.com League West Division against Burnley at Accrington Stanley FC.
Rovers, who included young professionals Craig Curran, Danny Holmes, Luke Waterfall, Paul Henry and Ryan Fraughan, were level at 0-0 at half time.
However, the home side made the most of some home defending to run in four second half goals to secure the win.
A disappointed Reserve Coach Shaun Garnett said: "It was a really poor second half with some schoolboy defending from the lads."
Tranmere: Collister, Holmes, Waterfall, Owens, Corrigan, Henry, Richardson, Fraughan (Denson 68), Macauley, Curran (Mackreth 60), Wilson. Substitutes not used: Kennedy, Rule.
