Tranmere Reserves will compete alongside eight other teams in the 2010-11 West Division of the totesport.com League.
Though Carlisle United, Rochdale and Blackpool have dropped out of the division from last season, Burnley, Bury and Macclesfield Town have all joined ahead of next season.
Staying in the division with Rovers are Accrington Stanley, Morecambe, Oldham Athletic, Preston North End and last season's winners, Wrexham.
The eight team Central Division will feature Barnsley, Bradford City, Derby County, Port Vale, Rotherham United, Sheffield United, Sheffield Wednesday and Shrewsbury Town, while Grimsby Town, Hartlepool United, Hull City, Gateshead, Leeds United, Lincoln City, Middlesbrough and Scunthorpe United will make up the East Division.
