Tranmere's Under-18 side will take on Farsley Celtic in the First Round of the FA Cup.
The Yorkshire side defeated Staveley Miners Welfare 5-3 on Monday night in the Third Qualifying Round to set up the tie at Prenton Park against Shaun Garnett's team.
It will be the first time the two sides have met in the FA Youth Cup.
The match will take place on Tuesday, 3 November, kick off 7.30pm. Admission is £3 for Adults, £2 Concessions.
